phrl-tk synthetic renilla luciferase reporter vector Search Results


renilla luciferase internal control vector  (Promega)
90
Promega renilla luciferase internal control vector
Renilla Luciferase Internal Control Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/renilla+luciferase+assay+system/pmc09617928-319-16-22
Average 90 stars, based on 1 article reviews
renilla luciferase internal control vector - by Bioz Stars, 2026-10
90/100 stars
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renilla luciferase internal control vector phrl-tk  (Promega)
90
Promega renilla luciferase internal control vector phrl-tk
Renilla Luciferase Internal Control Vector Phrl Tk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/pgl3+basic/10__1074_slash_jbc__ra119__010511-139-19-25
Average 90 stars, based on 1 article reviews
renilla luciferase internal control vector phrl-tk - by Bioz Stars, 2026-10
90/100 stars
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phrl-tk vector  (Promega)
90
Promega phrl-tk vector
Phrl Tk Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/phrl+tk+vector/10__1158_slash_1541___7786__mcr___21___0786-120-6-8
Average 90 stars, based on 1 article reviews
phrl-tk vector - by Bioz Stars, 2026-10
90/100 stars
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phrl-tk internal control plasmid  (Promega)
90
Promega phrl-tk internal control plasmid
Phrl Tk Internal Control Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/phrl+tk+internal+control+plasmid/us08298522-701-19-23
Average 90 stars, based on 1 article reviews
phrl-tk internal control plasmid - by Bioz Stars, 2026-10
90/100 stars
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phrl-tk  (Promega)
90
Promega phrl-tk
The PKM2 expression ( a , bottom), glucose uptake and lactate secretion ( a , top), OCR and ECAR ( b ) were detected. Norm.Unit represents the normalized OCR and ECAR ( b ). c Glucose uptake and lactate secretion was detected. d , e Proliferation and invasion ability were examined by proliferation assay ( d , n = 3 technical replicates, representative data from n = 3 independent experiments) and transwell invasion assay ( e ). f Tumor growth was examined using subcutaneous xenograft tumor model ( n = 6 in each group). The photograph (left) and weight (right) of tumors. g MYC TARGETS pathways were enriched in MYG1 highly expressed CRC patients (TCGA COADREAD cohort). h c-Myc expression was detected by western blot. The samples derive from the same experiment but different gels for c-Myc, and another for GAPDH were processed in parallel. i The expression of PKM2, c-Myc, MYG1, and GAPDH were detected by western blot. The samples derive from the same experiment but different gels for PKM2 and GAPDH, and another for c-Myc and MYG1 were processed in parallel. MYG1 mRNA was detected in cells with MYC koncked down ( j ) and MYC or TP53 overexpressed ( k , the samples derive from the same experiment but different gels for Flag, and another for GAPDH were processed in parallel). l – n SW620 ( m ) and LoVo ( n ) cell lysate were subjected to ChIP-qPCR. Schematic of primer pairs for qPCR ( l ). P13 was designed as a negative control and SOX2 was set as the positive control. o Schematic of fragments used for double <t>luciferase</t> report assay (top). Transcriptional activity of luciferase was detected and normalized by <t>Renilla</t> luciferase activity in 293 T cells (bottom). n = 3 independent experiments ( a – c , e , h – k , m– o ). Unpaired two-sided Student’s t- test ( m – n ). One-way ANOVA, Tukey’s multiple comparisons test ( a – c , f , and o ), Dunnett’s multiple comparisons test ( e , and j – k ). Two-way ANOVA, Tukey’s multiple comparisons test ( d ). Kolmogorov–Smirnov test ( g ). p value was provided in the figure. Error bars, mean ± SD. See also Supplementary Figs. and . Source data are provided as a Source Data file.
Phrl Tk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/phrl+tk/pmc11167044-577-27-32
Average 90 stars, based on 1 article reviews
phrl-tk - by Bioz Stars, 2026-10
90/100 stars
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phrl-tk- renilla luciferase  (Promega)
90
Promega phrl-tk- renilla luciferase
The PKM2 expression ( a , bottom), glucose uptake and lactate secretion ( a , top), OCR and ECAR ( b ) were detected. Norm.Unit represents the normalized OCR and ECAR ( b ). c Glucose uptake and lactate secretion was detected. d , e Proliferation and invasion ability were examined by proliferation assay ( d , n = 3 technical replicates, representative data from n = 3 independent experiments) and transwell invasion assay ( e ). f Tumor growth was examined using subcutaneous xenograft tumor model ( n = 6 in each group). The photograph (left) and weight (right) of tumors. g MYC TARGETS pathways were enriched in MYG1 highly expressed CRC patients (TCGA COADREAD cohort). h c-Myc expression was detected by western blot. The samples derive from the same experiment but different gels for c-Myc, and another for GAPDH were processed in parallel. i The expression of PKM2, c-Myc, MYG1, and GAPDH were detected by western blot. The samples derive from the same experiment but different gels for PKM2 and GAPDH, and another for c-Myc and MYG1 were processed in parallel. MYG1 mRNA was detected in cells with MYC koncked down ( j ) and MYC or TP53 overexpressed ( k , the samples derive from the same experiment but different gels for Flag, and another for GAPDH were processed in parallel). l – n SW620 ( m ) and LoVo ( n ) cell lysate were subjected to ChIP-qPCR. Schematic of primer pairs for qPCR ( l ). P13 was designed as a negative control and SOX2 was set as the positive control. o Schematic of fragments used for double <t>luciferase</t> report assay (top). Transcriptional activity of luciferase was detected and normalized by <t>Renilla</t> luciferase activity in 293 T cells (bottom). n = 3 independent experiments ( a – c , e , h – k , m– o ). Unpaired two-sided Student’s t- test ( m – n ). One-way ANOVA, Tukey’s multiple comparisons test ( a – c , f , and o ), Dunnett’s multiple comparisons test ( e , and j – k ). Two-way ANOVA, Tukey’s multiple comparisons test ( d ). Kolmogorov–Smirnov test ( g ). p value was provided in the figure. Error bars, mean ± SD. See also Supplementary Figs. and . Source data are provided as a Source Data file.
Phrl Tk Renilla Luciferase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/luciferase+assay+system/pmc10550242-181-14-19
Average 90 stars, based on 1 article reviews
phrl-tk- renilla luciferase - by Bioz Stars, 2026-10
90/100 stars
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renilla luciferase transfection control vector  (Promega)
90
Promega renilla luciferase transfection control vector
The PKM2 expression ( a , bottom), glucose uptake and lactate secretion ( a , top), OCR and ECAR ( b ) were detected. Norm.Unit represents the normalized OCR and ECAR ( b ). c Glucose uptake and lactate secretion was detected. d , e Proliferation and invasion ability were examined by proliferation assay ( d , n = 3 technical replicates, representative data from n = 3 independent experiments) and transwell invasion assay ( e ). f Tumor growth was examined using subcutaneous xenograft tumor model ( n = 6 in each group). The photograph (left) and weight (right) of tumors. g MYC TARGETS pathways were enriched in MYG1 highly expressed CRC patients (TCGA COADREAD cohort). h c-Myc expression was detected by western blot. The samples derive from the same experiment but different gels for c-Myc, and another for GAPDH were processed in parallel. i The expression of PKM2, c-Myc, MYG1, and GAPDH were detected by western blot. The samples derive from the same experiment but different gels for PKM2 and GAPDH, and another for c-Myc and MYG1 were processed in parallel. MYG1 mRNA was detected in cells with MYC koncked down ( j ) and MYC or TP53 overexpressed ( k , the samples derive from the same experiment but different gels for Flag, and another for GAPDH were processed in parallel). l – n SW620 ( m ) and LoVo ( n ) cell lysate were subjected to ChIP-qPCR. Schematic of primer pairs for qPCR ( l ). P13 was designed as a negative control and SOX2 was set as the positive control. o Schematic of fragments used for double <t>luciferase</t> report assay (top). Transcriptional activity of luciferase was detected and normalized by <t>Renilla</t> luciferase activity in 293 T cells (bottom). n = 3 independent experiments ( a – c , e , h – k , m– o ). Unpaired two-sided Student’s t- test ( m – n ). One-way ANOVA, Tukey’s multiple comparisons test ( a – c , f , and o ), Dunnett’s multiple comparisons test ( e , and j – k ). Two-way ANOVA, Tukey’s multiple comparisons test ( d ). Kolmogorov–Smirnov test ( g ). p value was provided in the figure. Error bars, mean ± SD. See also Supplementary Figs. and . Source data are provided as a Source Data file.
Renilla Luciferase Transfection Control Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/renilla+luciferase+transfection+efficiency+vector++phrltk/pmc02773194-428-29-35
Average 90 stars, based on 1 article reviews
renilla luciferase transfection control vector - by Bioz Stars, 2026-10
90/100 stars
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synthetic renilla luciferase reporter vector (phrl-tk  (Promega)
90
Promega synthetic renilla luciferase reporter vector (phrl-tk
The PKM2 expression ( a , bottom), glucose uptake and lactate secretion ( a , top), OCR and ECAR ( b ) were detected. Norm.Unit represents the normalized OCR and ECAR ( b ). c Glucose uptake and lactate secretion was detected. d , e Proliferation and invasion ability were examined by proliferation assay ( d , n = 3 technical replicates, representative data from n = 3 independent experiments) and transwell invasion assay ( e ). f Tumor growth was examined using subcutaneous xenograft tumor model ( n = 6 in each group). The photograph (left) and weight (right) of tumors. g MYC TARGETS pathways were enriched in MYG1 highly expressed CRC patients (TCGA COADREAD cohort). h c-Myc expression was detected by western blot. The samples derive from the same experiment but different gels for c-Myc, and another for GAPDH were processed in parallel. i The expression of PKM2, c-Myc, MYG1, and GAPDH were detected by western blot. The samples derive from the same experiment but different gels for PKM2 and GAPDH, and another for c-Myc and MYG1 were processed in parallel. MYG1 mRNA was detected in cells with MYC koncked down ( j ) and MYC or TP53 overexpressed ( k , the samples derive from the same experiment but different gels for Flag, and another for GAPDH were processed in parallel). l – n SW620 ( m ) and LoVo ( n ) cell lysate were subjected to ChIP-qPCR. Schematic of primer pairs for qPCR ( l ). P13 was designed as a negative control and SOX2 was set as the positive control. o Schematic of fragments used for double <t>luciferase</t> report assay (top). Transcriptional activity of luciferase was detected and normalized by <t>Renilla</t> luciferase activity in 293 T cells (bottom). n = 3 independent experiments ( a – c , e , h – k , m– o ). Unpaired two-sided Student’s t- test ( m – n ). One-way ANOVA, Tukey’s multiple comparisons test ( a – c , f , and o ), Dunnett’s multiple comparisons test ( e , and j – k ). Two-way ANOVA, Tukey’s multiple comparisons test ( d ). Kolmogorov–Smirnov test ( g ). p value was provided in the figure. Error bars, mean ± SD. See also Supplementary Figs. and . Source data are provided as a Source Data file.
Synthetic Renilla Luciferase Reporter Vector (Phrl Tk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/synthetic+renilla+luciferase+reporter+vector/10__1074_slash_jbc__m304199200-57-23-29
Average 90 stars, based on 1 article reviews
synthetic renilla luciferase reporter vector (phrl-tk - by Bioz Stars, 2026-10
90/100 stars
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normalization control vector (phrl-tk  (Promega)
90
Promega normalization control vector (phrl-tk
The PKM2 expression ( a , bottom), glucose uptake and lactate secretion ( a , top), OCR and ECAR ( b ) were detected. Norm.Unit represents the normalized OCR and ECAR ( b ). c Glucose uptake and lactate secretion was detected. d , e Proliferation and invasion ability were examined by proliferation assay ( d , n = 3 technical replicates, representative data from n = 3 independent experiments) and transwell invasion assay ( e ). f Tumor growth was examined using subcutaneous xenograft tumor model ( n = 6 in each group). The photograph (left) and weight (right) of tumors. g MYC TARGETS pathways were enriched in MYG1 highly expressed CRC patients (TCGA COADREAD cohort). h c-Myc expression was detected by western blot. The samples derive from the same experiment but different gels for c-Myc, and another for GAPDH were processed in parallel. i The expression of PKM2, c-Myc, MYG1, and GAPDH were detected by western blot. The samples derive from the same experiment but different gels for PKM2 and GAPDH, and another for c-Myc and MYG1 were processed in parallel. MYG1 mRNA was detected in cells with MYC koncked down ( j ) and MYC or TP53 overexpressed ( k , the samples derive from the same experiment but different gels for Flag, and another for GAPDH were processed in parallel). l – n SW620 ( m ) and LoVo ( n ) cell lysate were subjected to ChIP-qPCR. Schematic of primer pairs for qPCR ( l ). P13 was designed as a negative control and SOX2 was set as the positive control. o Schematic of fragments used for double <t>luciferase</t> report assay (top). Transcriptional activity of luciferase was detected and normalized by <t>Renilla</t> luciferase activity in 293 T cells (bottom). n = 3 independent experiments ( a – c , e , h – k , m– o ). Unpaired two-sided Student’s t- test ( m – n ). One-way ANOVA, Tukey’s multiple comparisons test ( a – c , f , and o ), Dunnett’s multiple comparisons test ( e , and j – k ). Two-way ANOVA, Tukey’s multiple comparisons test ( d ). Kolmogorov–Smirnov test ( g ). p value was provided in the figure. Error bars, mean ± SD. See also Supplementary Figs. and . Source data are provided as a Source Data file.
Normalization Control Vector (Phrl Tk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/normalization+control+vector++phrl+tk/10__1074_slash_jbc__m113__460287-76-27-31
Average 90 stars, based on 1 article reviews
normalization control vector (phrl-tk - by Bioz Stars, 2026-10
90/100 stars
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ptarget vector  (Promega)
90
Promega ptarget vector
The PKM2 expression ( a , bottom), glucose uptake and lactate secretion ( a , top), OCR and ECAR ( b ) were detected. Norm.Unit represents the normalized OCR and ECAR ( b ). c Glucose uptake and lactate secretion was detected. d , e Proliferation and invasion ability were examined by proliferation assay ( d , n = 3 technical replicates, representative data from n = 3 independent experiments) and transwell invasion assay ( e ). f Tumor growth was examined using subcutaneous xenograft tumor model ( n = 6 in each group). The photograph (left) and weight (right) of tumors. g MYC TARGETS pathways were enriched in MYG1 highly expressed CRC patients (TCGA COADREAD cohort). h c-Myc expression was detected by western blot. The samples derive from the same experiment but different gels for c-Myc, and another for GAPDH were processed in parallel. i The expression of PKM2, c-Myc, MYG1, and GAPDH were detected by western blot. The samples derive from the same experiment but different gels for PKM2 and GAPDH, and another for c-Myc and MYG1 were processed in parallel. MYG1 mRNA was detected in cells with MYC koncked down ( j ) and MYC or TP53 overexpressed ( k , the samples derive from the same experiment but different gels for Flag, and another for GAPDH were processed in parallel). l – n SW620 ( m ) and LoVo ( n ) cell lysate were subjected to ChIP-qPCR. Schematic of primer pairs for qPCR ( l ). P13 was designed as a negative control and SOX2 was set as the positive control. o Schematic of fragments used for double <t>luciferase</t> report assay (top). Transcriptional activity of luciferase was detected and normalized by <t>Renilla</t> luciferase activity in 293 T cells (bottom). n = 3 independent experiments ( a – c , e , h – k , m– o ). Unpaired two-sided Student’s t- test ( m – n ). One-way ANOVA, Tukey’s multiple comparisons test ( a – c , f , and o ), Dunnett’s multiple comparisons test ( e , and j – k ). Two-way ANOVA, Tukey’s multiple comparisons test ( d ). Kolmogorov–Smirnov test ( g ). p value was provided in the figure. Error bars, mean ± SD. See also Supplementary Figs. and . Source data are provided as a Source Data file.
Ptarget Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/ptarget+vector/10__1074_slash_jbc__m115__699363-29-3-15
Average 90 stars, based on 1 article reviews
ptarget vector - by Bioz Stars, 2026-10
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renilla luciferase vector phrl-tk  (SwitchGear Genomics)
90
SwitchGear Genomics renilla luciferase vector phrl-tk
The PKM2 expression ( a , bottom), glucose uptake and lactate secretion ( a , top), OCR and ECAR ( b ) were detected. Norm.Unit represents the normalized OCR and ECAR ( b ). c Glucose uptake and lactate secretion was detected. d , e Proliferation and invasion ability were examined by proliferation assay ( d , n = 3 technical replicates, representative data from n = 3 independent experiments) and transwell invasion assay ( e ). f Tumor growth was examined using subcutaneous xenograft tumor model ( n = 6 in each group). The photograph (left) and weight (right) of tumors. g MYC TARGETS pathways were enriched in MYG1 highly expressed CRC patients (TCGA COADREAD cohort). h c-Myc expression was detected by western blot. The samples derive from the same experiment but different gels for c-Myc, and another for GAPDH were processed in parallel. i The expression of PKM2, c-Myc, MYG1, and GAPDH were detected by western blot. The samples derive from the same experiment but different gels for PKM2 and GAPDH, and another for c-Myc and MYG1 were processed in parallel. MYG1 mRNA was detected in cells with MYC koncked down ( j ) and MYC or TP53 overexpressed ( k , the samples derive from the same experiment but different gels for Flag, and another for GAPDH were processed in parallel). l – n SW620 ( m ) and LoVo ( n ) cell lysate were subjected to ChIP-qPCR. Schematic of primer pairs for qPCR ( l ). P13 was designed as a negative control and SOX2 was set as the positive control. o Schematic of fragments used for double <t>luciferase</t> report assay (top). Transcriptional activity of luciferase was detected and normalized by <t>Renilla</t> luciferase activity in 293 T cells (bottom). n = 3 independent experiments ( a – c , e , h – k , m– o ). Unpaired two-sided Student’s t- test ( m – n ). One-way ANOVA, Tukey’s multiple comparisons test ( a – c , f , and o ), Dunnett’s multiple comparisons test ( e , and j – k ). Two-way ANOVA, Tukey’s multiple comparisons test ( d ). Kolmogorov–Smirnov test ( g ). p value was provided in the figure. Error bars, mean ± SD. See also Supplementary Figs. and . Source data are provided as a Source Data file.
Renilla Luciferase Vector Phrl Tk, supplied by SwitchGear Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/renilla+luciferase/pmc06052162-91-18-27
Average 90 stars, based on 1 article reviews
renilla luciferase vector phrl-tk - by Bioz Stars, 2026-10
90/100 stars
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phrl-tk-backbone plasmid  (Promega)
90
Promega phrl-tk-backbone plasmid
The PKM2 expression ( a , bottom), glucose uptake and lactate secretion ( a , top), OCR and ECAR ( b ) were detected. Norm.Unit represents the normalized OCR and ECAR ( b ). c Glucose uptake and lactate secretion was detected. d , e Proliferation and invasion ability were examined by proliferation assay ( d , n = 3 technical replicates, representative data from n = 3 independent experiments) and transwell invasion assay ( e ). f Tumor growth was examined using subcutaneous xenograft tumor model ( n = 6 in each group). The photograph (left) and weight (right) of tumors. g MYC TARGETS pathways were enriched in MYG1 highly expressed CRC patients (TCGA COADREAD cohort). h c-Myc expression was detected by western blot. The samples derive from the same experiment but different gels for c-Myc, and another for GAPDH were processed in parallel. i The expression of PKM2, c-Myc, MYG1, and GAPDH were detected by western blot. The samples derive from the same experiment but different gels for PKM2 and GAPDH, and another for c-Myc and MYG1 were processed in parallel. MYG1 mRNA was detected in cells with MYC koncked down ( j ) and MYC or TP53 overexpressed ( k , the samples derive from the same experiment but different gels for Flag, and another for GAPDH were processed in parallel). l – n SW620 ( m ) and LoVo ( n ) cell lysate were subjected to ChIP-qPCR. Schematic of primer pairs for qPCR ( l ). P13 was designed as a negative control and SOX2 was set as the positive control. o Schematic of fragments used for double <t>luciferase</t> report assay (top). Transcriptional activity of luciferase was detected and normalized by <t>Renilla</t> luciferase activity in 293 T cells (bottom). n = 3 independent experiments ( a – c , e , h – k , m– o ). Unpaired two-sided Student’s t- test ( m – n ). One-way ANOVA, Tukey’s multiple comparisons test ( a – c , f , and o ), Dunnett’s multiple comparisons test ( e , and j – k ). Two-way ANOVA, Tukey’s multiple comparisons test ( d ). Kolmogorov–Smirnov test ( g ). p value was provided in the figure. Error bars, mean ± SD. See also Supplementary Figs. and . Source data are provided as a Source Data file.
Phrl Tk Backbone Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrl-tk+synthetic+renilla+luciferase+reporter+vector/phrl+tk+plasmid/pmc03738543-33-5-14
Average 90 stars, based on 1 article reviews
phrl-tk-backbone plasmid - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


The PKM2 expression ( a , bottom), glucose uptake and lactate secretion ( a , top), OCR and ECAR ( b ) were detected. Norm.Unit represents the normalized OCR and ECAR ( b ). c Glucose uptake and lactate secretion was detected. d , e Proliferation and invasion ability were examined by proliferation assay ( d , n = 3 technical replicates, representative data from n = 3 independent experiments) and transwell invasion assay ( e ). f Tumor growth was examined using subcutaneous xenograft tumor model ( n = 6 in each group). The photograph (left) and weight (right) of tumors. g MYC TARGETS pathways were enriched in MYG1 highly expressed CRC patients (TCGA COADREAD cohort). h c-Myc expression was detected by western blot. The samples derive from the same experiment but different gels for c-Myc, and another for GAPDH were processed in parallel. i The expression of PKM2, c-Myc, MYG1, and GAPDH were detected by western blot. The samples derive from the same experiment but different gels for PKM2 and GAPDH, and another for c-Myc and MYG1 were processed in parallel. MYG1 mRNA was detected in cells with MYC koncked down ( j ) and MYC or TP53 overexpressed ( k , the samples derive from the same experiment but different gels for Flag, and another for GAPDH were processed in parallel). l – n SW620 ( m ) and LoVo ( n ) cell lysate were subjected to ChIP-qPCR. Schematic of primer pairs for qPCR ( l ). P13 was designed as a negative control and SOX2 was set as the positive control. o Schematic of fragments used for double luciferase report assay (top). Transcriptional activity of luciferase was detected and normalized by Renilla luciferase activity in 293 T cells (bottom). n = 3 independent experiments ( a – c , e , h – k , m– o ). Unpaired two-sided Student’s t- test ( m – n ). One-way ANOVA, Tukey’s multiple comparisons test ( a – c , f , and o ), Dunnett’s multiple comparisons test ( e , and j – k ). Two-way ANOVA, Tukey’s multiple comparisons test ( d ). Kolmogorov–Smirnov test ( g ). p value was provided in the figure. Error bars, mean ± SD. See also Supplementary Figs. and . Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration

doi: 10.1038/s41467-024-49221-0

Figure Lengend Snippet: The PKM2 expression ( a , bottom), glucose uptake and lactate secretion ( a , top), OCR and ECAR ( b ) were detected. Norm.Unit represents the normalized OCR and ECAR ( b ). c Glucose uptake and lactate secretion was detected. d , e Proliferation and invasion ability were examined by proliferation assay ( d , n = 3 technical replicates, representative data from n = 3 independent experiments) and transwell invasion assay ( e ). f Tumor growth was examined using subcutaneous xenograft tumor model ( n = 6 in each group). The photograph (left) and weight (right) of tumors. g MYC TARGETS pathways were enriched in MYG1 highly expressed CRC patients (TCGA COADREAD cohort). h c-Myc expression was detected by western blot. The samples derive from the same experiment but different gels for c-Myc, and another for GAPDH were processed in parallel. i The expression of PKM2, c-Myc, MYG1, and GAPDH were detected by western blot. The samples derive from the same experiment but different gels for PKM2 and GAPDH, and another for c-Myc and MYG1 were processed in parallel. MYG1 mRNA was detected in cells with MYC koncked down ( j ) and MYC or TP53 overexpressed ( k , the samples derive from the same experiment but different gels for Flag, and another for GAPDH were processed in parallel). l – n SW620 ( m ) and LoVo ( n ) cell lysate were subjected to ChIP-qPCR. Schematic of primer pairs for qPCR ( l ). P13 was designed as a negative control and SOX2 was set as the positive control. o Schematic of fragments used for double luciferase report assay (top). Transcriptional activity of luciferase was detected and normalized by Renilla luciferase activity in 293 T cells (bottom). n = 3 independent experiments ( a – c , e , h – k , m– o ). Unpaired two-sided Student’s t- test ( m – n ). One-way ANOVA, Tukey’s multiple comparisons test ( a – c , f , and o ), Dunnett’s multiple comparisons test ( e , and j – k ). Two-way ANOVA, Tukey’s multiple comparisons test ( d ). Kolmogorov–Smirnov test ( g ). p value was provided in the figure. Error bars, mean ± SD. See also Supplementary Figs. and . Source data are provided as a Source Data file.

Article Snippet: Fragment 1 (F1) and fragment 2 (F2) of MYG1 promoter were cloned into double-digested pGL3-Basic with KpnI and EcoRI. pGL3 vectors were subsequently co-transfected with the active reporter renilla luciferase vector phRL-TK (Promega) into 293 T cells.

Techniques: Expressing, Proliferation Assay, Transwell Invasion Assay, Western Blot, Negative Control, Positive Control, Luciferase, Activity Assay